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Development of MS-based analytical methods for glycomics & glycoproteomics

Information on composition and structure of glycans, glycan heterogeneity, and glycosite occupancy is increasingly recognized as crucial to understanding glycan and glycoprotein structure and function in a biological system. Yet, no sensitive approach currently exists for the analysis of glycome and glycoproteome despite a biological importance of glycosylation. The AGRS has developed new and improved analytical techniques for comprehensive glycan and glycosite profiling using high performance mass spectrometry that can be applied in glycoproteomics and glycomic research.

Research highlights:
  • Efficient and selective method to capture O-glycans from complex mixture
  • Isomer-specific glycan separation and quantitation
  • Selective enrichment of phosphoglycans using newly designed LC/MS chip
Relevant publications:
  • Hua, S., Hu, C.Y., Kim, B.J., Totten, S.M., Oh, M.J., Yun, N.Y., Nwosu, C.C., Yoo, J.S., Lebrilla, C.B., An, H.J., “Glyco-Analytical Multispecific Proteolysis (Glyco-AMP): A Simple Method for Detailed and Quantitative Glycoproteomic Characterization”, Journal of Proteome Research, 2013, 12(10), 4414-23.
  • Strum, J.S., Nwosu, C.C., Hua, S., Kronewitter, S.R., Seipert, R.R., Bachelor, R.J., An, H.J., Lebrilla, C.B., “Automated Assignments of N- and O-Site Specific Glycosylation with Extensive Glycan Heterogeneity of Glycoprotein Mixtures”, Analytical Chemistry, 2013, 85, 5666−5675.
  • Hua, S., Oh, M.J., An, H.J., “Multi-Level Characterization of Protein Glycosylation”, Mass Spectrometry Letters, 2013, 4(1), 10–17
  • Nwosu, C.C., Seipert, R.R., Strum, J.S., Hua, S., An, H.J., Zivkovic, A.M., German, B.J., Lebrilla, C.B., “Simultaneous and Extensive Site-specific N- and O-Glycosylation Analysis in Protein Mixtures”, Journal of Proteome Research, 2011, 10, 2612–2624.
  • Hua, S., Lebrilla, C.B., An, H.J., “Application of nano-LC-based glycomics towards biomarker discovery”, Bioanalysis, 2011, 3(22), 2573–2585.