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Glycosylation plays an important role in ensuring the proper structure and function of most biotherapeutic proteins. Even small changes in glycan composition, structure, or location can have a drastic impact on drug safety and efficacy. The AGRS works with biopharmaceutical companies as well as government regulatory agencies to create platforms for accurate and detailed analysis of biopharmaceutical protein glycosylation.

Research highlights:
  • Multi-level glycomic characterization of biotherapeutics from intact protein to native glycan
  • Isomer-specific structural characterization of N- and O-glycans
  • Comprehensive identification and quantitation of glycan modifications such as O-acetylation, polylactosaminylation, dehydration, phosphorylation, etc.
  • Characterization of non-human glycans related to immunogenicity.
  • Development of cell glycan phenotyping technology for quality evaluation of cell therapeutics.
  • Establishment of AI technology based platform for comparability assessment and quality verification of antibody biosimilars.
Relevant publications:
  • Oh, M.J., Seo, Y., Kim, U., An, H.J., “In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC SPE and LC-MS/MS”, Methods in Molecular Biology (Clifton, NJ), 2021, 2271, 121-131.
  • De Leoz, M.L.A, Duewer, D.L, Fung, A., Liu, L., … Seo, Y., An, H.J., et al. “NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods”, Molecular & Cellular Proteomics, 2020, 19(1), 11-30.
  • Seo, Y., Oh, M.J., Park, J.Y., Ko, J.K., Kim, J.Y., An, H.J., “Comprehensive Characterization of Biotherapeutics by Selective Capturing of Highly Acidic Glycans using Stepwise PGC-SPE and LC/MS/MS”, Analytical chemistry, 2019, 91(9), 6064-6071.
  • Lee, H.H., Heo, C.E., Seo, N., Yun, S.G, An, H.J., Kim H.I. “Accurate Quantification of N-Glycolylneuraminic Acid in Therapeutic Proteins Using Supramolecular Mass Spectrometry”,  Journal of the American Chemical Society, 2018, 140(48), 16528-16534.
  • Seo, Y., Park, G.M., Oh, M.J., An, H.J., “Investigation of O-glycosylation Heterogeneity of Recombinant Coagulation Factor IX using LC/MS/MS ”, Bioanalysis, 2017, 9(18), 1361-1372.
  • Kim, U., Oh, M.J., Seo, Y., Jeon, Y., Eom, J.H., An, H.J., “Sensitive and Comprehensive Analysis of O-glycosylation in Biotherapeutics: A Case Study of NESP”, Bioanalysis, 2017, 9(18), 1373-1383.
  • Oh, M.J., Hua, S., Kim, U., Kim, H.J., Lee, J., Kim, J.H., An, H.J., “Analytical detection and characterization of biopharmaceutical glycosylation by MS”, Bioanalysis, 2016, 8(7), 711-727.
  • Hua, S., Oh, M.J., Ozcan, S., Seo, Y., Grimm, R., An, H.J., “Technologies for glycomic characterization of biopharmaceutical erythropoietins”, TrAC Trends in Analytical Chemistry, 2015, 68, 18-27, 18-27.
  • Seo, Y.. Kim, U., Oh, M.J., Yun, N., An, H.J., “MS Platform for Erythropoietin Glycome Characterization”, Mass Spectrometry Letters, 2015, 6(3), 53-58.