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Facilities-Instruments

mass spectrometer

Agilent 6540 Accurate Mass Nano LC-chip
Q-TOF MS system
Application

Profiling, identifying, characterizing, and quantifying low molecular weight compounds and biomolecules.

  • Characterization of glycome variants in glycoprotein biotherapeutics
  • Development of novel chip: TiO2-PGC chip for glycome separation
  • Sugar survival: Glycosylation in ancient mummies
  • Forensic analytical science & technology: Post-mortem interval (PMI) biomarker discovery
Specification
Sensitivity 1 pg
Mass accuracy 1 ppm (MS) / 2 ppm (MS/MS)
Mass resolution 25,000 at 322 m/z / 42,000 at 2,722 m/z
Dynamic range 5.0 orders
Mass range m/z 100 - 10,000
Tandem MS CID, MS2
Detector High-energy conversion dynode and high-gain electron multiplier horn

Bruker Ultraflextreme MALDI-TOF/TOF MS
Application
  • Rapid profiling of glycan and glycopeptide
  • Identification of microorganism by biotyping
  • Elucidation of glycan structure using high energy CID
  • Determination of intact (glyco)protein molecular weight
Specification
Sensitivity Low pmol
Mass accuracy 1 ppm
Mass resolution > 40,000 (on peptide mass range)
Dynamic range -
Mass range Low : ~10,000 / Medium : ~100,000 / High : ~500,000 m/z
Laser Smartbeam-II (Nd:YAG), 1~1000Hz
Tandem MS High energy CID, TOF/TOF
Detector FlashDetector (microchannel plate)

Agilent 6530 Accurate Mass Nano LC-chip
Q-TOF MS System
Application

Profiling, identifying, characterizing, and quantifying low molecular weight compounds and biomolecules.

  • Glycome characterization of biotherapeutics
  • Biomarker discovery for cancer and infectious disease diagnosis
  • Alteration of glycosylation during natural aging
  • Development of analytical tools for extraction and purification of glycome from biological samples
Specification
Sensitivity 1 pg; 10:1 S/N in MS mode / 1 pg; 50:1 S/N in MS/MS mode
Mass accuracy <2 ppm MS / < 5 ppm MS/MS
Mass resolution 10,000 @ 118 m/z / 20,000 @ 1522 m/z
Dynamic range 5.0 orders
Mass range 8,000 m/z quad (opt) / 20,000 m/z TOF
Tandem MS CID, MS2
Detector MCP (microchannel plate)

Agilent 6460 UPLC Triple Quadrupole MS System
Application
  • Rapid screening and qualitative analysis for glycans and metabolites
  • Absolute quantitative analysis for small molecules using dynamic MRM
  • Environmental and food component analysis
Specification
Sensitivity 1pg
Mass accuracy 0.1 Dalton (5-1,000m/z)
Mass resolution 0.5~0.7 Dalton (tune)
Dynamic range >6.0 x 106
Mass range 5-3,000 m/z
Tandem MS Scan, SIM, PIS, MRM
Detector Electron Multiplier

Agilent 1260 UHPLC
Application
  • Absolute quantitative analysis for small molecules and biomolecules
  • Pharmaceutical and food component analysis
Specification
Pump 1260 binary pump
Max. Pressure up to 600bar
Flow rate up to 5mL/min
Delay volumes down to 120 μL
Suitable column from 1.8μm, 2.1mm I.D. to 4.6μm, 3mm I.D.
Detector 1260 VWD

Agilent 1290 Infinity Ⅱ 2D-LC
Application
  • High-resolution separation and analysis of highly complex biological samples such as glycopeptides and glycolipids.
  • Unraveling the complexity of glycan isomers
  • Simultaneous qualitative & quantitative analysis of biological samples
Specification
1D, 2D pump 1290 High Speed Pump
Function Comprehensive & heart-cutting 2D-LC mode
Pressure up to 1300bar
Flow rate up to 5mL/min
Lowest delay volumes to 10 μL
Autosampler 1290 MCT
Detector 1290 DAD FS, 1290 DAD, 1260 FLD Spectra

for sample preparation

  • Cell sonicator (Qsonica)
  • Ultracentrifuge (Beckman Coulter)
  • Microwave reactor
    (Hudson Surface Technology)
  • Thermomixer(Eppendorf)
  • Refrigerated Centrifuge (Eppendorf)
  • EZ-2 personal evaporator (Genevac)
  • Bravo Automated Liquid Handling Platform
    with BenchCel Microplate Handler
    (Agilent technologies)
  • Immunoaffinity
  • Solid Phase Extraction with a Porous
    Graphitized Carbon Cartridge

Facilities-Laboratory

#342 / Asia-Pacific Glycomics Reference Site

#438 / GLYCOME Imaging Lab

#440 / Laboratory for Sample Preparation

#448 / Asia Glycomics Reference Site